Review Article

Strong Linkage Disequilibrium Between -670 A>G and -1377 G>A Polymorphisms in FAS Gene in Patients with Breast Cancer and Controls

Maryam Davarpanah Tanha Ghochan, Mojgan Mohammadi , Amir Jalali, Jalil Tavakolafshari, Fatemeh Homaei shandiz, Amin Reza Nikpoor

Maryam Davarpanah Tanha Ghochan
Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran

Mojgan Mohammadi
Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran. Email: Mohammadimzh@mums.ac.ir

Amir Jalali
Department of Immunology, Medical school, Shahid beheshti University of Medical Science, Tehran, Iran

Jalil Tavakolafshari
Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran

Fatemeh Homaei shandiz
Surgical Oncology Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Amin Reza Nikpoor

Online First: August 13, 2016 | Cite this Article
Davarpanah Tanha Ghochan, M., Mohammadi, M., Jalali, A., Tavakolafshari, J., Homaei shandiz, F., Nikpoor, A. 2016. Strong Linkage Disequilibrium Between -670 A>G and -1377 G>A Polymorphisms in FAS Gene in Patients with Breast Cancer and Controls. The Cancer Press 2(3): 55-58. DOI:10.15562/tcp.19


Breast cancer is the second cause of mortality in women. The etiology of breast cancer is multi factorial. Genetic factors play an important role in the etimology of breast cancer. The FAS gene, has a critical role in the tumor growth and metastasis. Gene polymorphisms including -1377 G>A  and -670 A/G in FAS gene have shown to change the transcription activities of this gene. The FAS genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 115 breast cancer patients, 115 healthy controls, all female and selected from city of Mashhad in north-eastern part of Iran. BstuI and ScrfI were used  as endonuclease enzymes to detect -1377G/A and  -670A/G gene polymorphisms, respectively. GG, GA and AA genotype frequencies for FAS -1377 polymorphism in patients  were   30.4%, 49.6% and 20%, whereas in healthy controls were 26.1%, 50.4% and 23.5%, respectively and there was no significant difference between case and controls (p=0.7).Additionally, genotype frequencies of FAS -670 AA, AG and GG in patient groups were 52.2%, 39.1% and 8.7% respectively . The result of genotype frequency in controls for FAS -670 AA, AG and GG was 47%, 41% and 10.4%, which Showed insignificant difference as compared with patients genotypes (p=0.78). These results showed that FAS−1377G/A and FAS -670 A/G gene polymorphisms had a strong linkage disequilibrium with  p value <0.001. These results indicated the lack of association between FAS−1377G>A and FAS -670 A/G gene polymorphisms and risk of breast cancer. Moreover, a significant linkage disequilibrium was found betweenFAS−1377G/A and FAS -670 A/G gene polymorphisms in case and control groups.

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